Different Methods for DNA Purification

DNA purification is a standard and important procedure in molecular biology. The purpose of DNA purification is to isolate the desired genetic material from the contaminant (proteins, cells membranes and RNA). This is an essential procedure in nearly all molecular processes and must be done correctly in order in order to obtain top-quality, usable DNA.

There are many different approaches for DNA purification. The selection of the method is contingent on a myriad of factors like the source materials, downstream applications, cost and time limitations. The typical genomic and plasmid purification procedures include chemical treatment, enzyme digestion or mechanical disintegration of cells and tissues, and then salting out the proteins and removing the DNA using alcohol.

Ethanol precipitation can be a low-cost simple, quick and easy method of desalting and concentrating DNA. DNA molecules form aggregates purchase science supplies when they are in the presence of monovalent cations such sodium, and then are filtered out of solution with large amounts of ethanol. This technique permits the removal of organic compounds and other impurities from the sample and is frequently used in conjunction with other purification techniques.

Anion exchange chromatography is another popular method of DNA purification. The interaction between the negatively-charged DNA phosphate backbones as well as the positively-charged surface molecules of resins bind DNA in a solvent and positively charged resins. During the binding process it is possible to remove contaminants making use of a strict washing process. The DNA that has been purified is eluted under low salt conditions.